2g1p

X-ray diffraction
1.89Å resolution

Structure of E. coli DNA adenine methyltransferase (DAM)

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA adenine methylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 32.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE8 (Residues: 1-278; Coverage: 100%)
Gene names: JW3350, b3387, dam
Sequence domains: D12 class N6 adenine-specific DNA methyltransferase
Structure domains:
5'-D(*TP*CP*TP*AP*GP*AP*TP*CP*TP*AP*GP*A)-3' Chains: F, G
Molecule details ›
Chains: F, G
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 46.2Å b: 71.3Å c: 97.8Å
α: 90° β: 90.03° γ: 90°
R-values:
R R work R free
0.188 0.188 0.216
Expression systems:
  • Escherichia coli
  • Not provided