› 2g1k

X-ray diffraction
1.75Å resolution

Crystal structure of Mycobacterium tuberculosis shikimate kinase in complex with shikimate at 1.75 angstrom resolution

Released:
DOI: 10.2210/pdb2g1k/pdb
PDB 2g1k coloured by chain and viewed from the front.
PDB 2g1k coloured by chain and viewed from the side.
PDB 2g1k coloured by chain and viewed from the top.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Mycobacterium tuberculosis shikimate kinase in complex with shikimic acid and an ATP analogue.
Gan J, Gu Y, Li Y, Yan H, Ji X
Biochemistry 45 8539-45 (2006)
PMID: 16834327

Function and Biology Details

Reaction catalysed: 
ATP + shikimate = ADP + shikimate 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate kinase Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 18.61 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPY3 (Residues: 1-176; Coverage: 100%)
Gene names: MTCY159.17, Rv2539c, aroK
Sequence domains: Shikimate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand CL 2 x CL
Ligand SKM 1 x SKM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 59.309Å b: 59.309Å c: 102.537Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.222 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The shikimate pathway and aromatic amino Acid biosynthesis in plants.
Maeda et al. (2012)
21 other articles

2 mentions without citation

Selective Mycobacterium tuberculosis Shikimate Kinase Inhibitors as Potential Antibacterials.
Gordon et al. (2015)
1 more