› 2fzu

X-ray diffraction
1.25Å resolution

Reduced enolate chromophore intermediate for GFP variant

Released:
DOI: 10.2210/pdb2fzu/pdb
PDB 2fzu coloured by chain and viewed from the front.
PDB 2fzu coloured by chain and viewed from the side.
PDB 2fzu coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Structural evidence for an enolate intermediate in GFP fluorophore biosynthesis.
Barondeau DP, Tainer JA, Getzoff ED
J. Am. Chem. Soc. 128 3166-8 (2006)
PMID: 16522096

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.82 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand EDO 1 x EDO
1 modified residue:
Ligand C12 1 x C12

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 51.35Å b: 62.52Å c: 71.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.142 0.199
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Excited state reactions in fluorescent proteins.
Meech SR. (2009)
4 more