› 2fyf

X-ray diffraction
1.5Å resolution

Structure of a putative phosphoserine aminotransferase from Mycobacterium Tuberculosis

Released:
DOI: 10.2210/pdb2fyf/pdb
PDB entry 2fyf coloured by chain, front view.
PDB entry 2fyf coloured by chain, side view.
PDB entry 2fyf coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of phosphoserine aminotransferase from Mycobacterium tuberculosis.
Coulibaly F, Lassalle E, Baker HM, Baker EN
Acta Crystallogr D Biol Crystallogr 68 553-63 (2012)
PMID: 22525753

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 42.97 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQ73 (Residues: 1-376; Coverage: 100%)
Gene names: MTCY31.12c, Rv0884c, serC
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
3 bound ligands:
Ligand SO4 2 x SO4
Ligand PC4 6 x PC4
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 77.478Å b: 94.056Å c: 101.063Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.166
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

High throughput screen identifies small molecule inhibitors specific for Mycobacterium tuberculosis phosphoserine phosphatase.
Arora et al. (2014)
4 more

1 mention without citation

Targeting the Serine Pathway: A Promising Approach against Tuberculosis?
Haufroid et al. (2019)