X-ray diffraction
1.4Å resolution

Reduced enolate chromophore intermediate for Y66H GFP variant

Source organism: Aequorea victoria
Primary publication:
Structural evidence for an enolate intermediate in GFP fluorophore biosynthesis.
J. Am. Chem. Soc. 128 3166-8 (2006)
PMID: 16522096

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.73 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 51.135Å b: 62.451Å c: 71.453Å
α: 90° β: 90° γ: 90°
R R work R free
0.136 not available 0.198
Expression system: Escherichia coli BL21(DE3)