› 2fue

X-ray diffraction
1.75Å resolution

Human alpha-Phosphomannomutase 1 with D-mannose 1-phosphate and Mg2+ cofactor bound

Released:
DOI: 10.2210/pdb2fue/pdb
PDB 2fue coloured by chain and viewed from the front.
PDB 2fue coloured by chain and viewed from the side.
PDB 2fue coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
The X-ray crystal structures of human alpha-phosphomannomutase 1 reveal the structural basis of congenital disorder of glycosylation type 1a.
Silvaggi NR, Zhang C, Lu Z, Dai J, Dunaway-Mariano D, Allen KN
J. Biol. Chem. 281 14918-26 (2006)
PMID: 16540464

Function and Biology Details

Reaction catalysed: 
Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphomannomutase 1 Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 29.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92871 (Residues: 1-262; Coverage: 100%)
Gene names: PMM1, PMMH22
Sequence domains: Eukaryotic phosphomannomutase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand M1P 1 x M1P
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P43212
Unit cell:
a: 51.39Å b: 51.39Å c: 214.603Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.24
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Congenital disorders of glycosylation: an update on defects affecting the biosynthesis of dolichol-linked oligosaccharides.
Haeuptle et al. (2009)
2 more

8 mentions without citation

Beyond structural genomics: computational approaches for the identification of ligand binding sites in protein structures.
Ghersi et al. (2011)
7 more