› 2fss

X-ray diffraction
1.7Å resolution

Candida boidinii formate dehydrogenase (FDH) K47E mutant

Released:
DOI: 10.2210/pdb2fss/pdb
PDB 2fss coloured by chain and viewed from the front.
PDB 2fss coloured by chain and viewed from the side.
PDB 2fss coloured by chain and viewed from the top.
Source organism: [Candida] boidinii
Primary publication:
High-resolution structures of formate dehydrogenase from Candida boidinii.
Schirwitz K, Schmidt A, Lamzin VS
Protein Sci. 16 1146-56 (2007)
PMID: 17525463

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 365 amino acids
Theoretical weight: 40.39 KDa
Source organism: [Candida] boidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: O13437 (Residues: 2-364; Coverage: 100%)
Gene names: FDH, FDH1, FDH3
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P1
Unit cell:
a: 53.43Å b: 68.389Å c: 109.369Å
α: 77.93° β: 89.33° γ: 81.34°
R-values:
R R work R free
0.206 0.204 0.249
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase.
Stripp et al. (2022)
31 other articles

5 mentions without citation

High-resolution structures of formate dehydrogenase from Candida boidinii.
Schirwitz et al. (2007)
4 more