› 2fsa

X-ray diffraction
1.9Å resolution

Crystal structure of PHD finger-linker-bromodomain fragment of human BPTF in the H3(1-15)K4ME2 bound state

Released:
DOI: 10.2210/pdb2fsa/pdb
PDB entry 2fsa coloured by chain, front view.
PDB entry 2fsa coloured by chain, side view.
PDB entry 2fsa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF.
Li H, Ilin S, Wang W, Duncan EM, Wysocka J, Allis CD, Patel DJ
Nature 442 91-5 (2006)
PMID: 16728978

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-159403 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleosome-remodeling factor subunit BPTF Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 174 amino acids
Theoretical weight: 20.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12830 (Residues: 2865-3033; Coverage: 6%)
Gene names: BPTF, FAC1, FALZ
Sequence domains:
Structure domains:
Histone H3.1 Chain: P
Molecule details ›
Chain: P
Length: 15 amino acids
Theoretical weight: 1.59 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-16; Coverage: 11%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
2 modified residues:
Ligand MSE 15 x MSE
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 38.451Å b: 85.169Å c: 109.567Å
α: 90° β: 99.03° γ: 90°
R-values:
R R work R free
0.2 0.2 0.22
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

149 review citations

The epigenomics of cancer.
Jones et al. (2007)
148 more

7 mentions without citation

Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF.
Li et al. (2006)
6 more