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Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain.

Drake EJ, Nicolai DA, Gulick AM
Chem Biol 13 409-19 (2006)
Cited: 72 times
EuropePMC logo PMID: 16632253

Abstract

Nonribosomal peptide synthetases are modular proteins that operate in an assembly line fashion to bind, modify, and link amino acids. In the E. coli enterobactin NRPS system, the EntE adenylation domain catalyzes the transfer of a molecule of 2,3-dihydroxybenzoic acid to the pantetheine cofactor of EntB. We present here the crystal structure of the EntB protein that contains an N-terminal isochorismate lyase domain that functions in the synthesis of 2,3-dihydroxybenzoate and a C-terminal carrier protein domain. Functional analysis showed that the EntB-EntE interaction was surprisingly tolerant of a number of point mutations on the surface of EntB and EntE. Mutational studies on EntE support our previous hypothesis that members of the adenylate-forming family of enzymes adopt two distinct conformations to catalyze the two-step reactions.

Reviews - 2fq1 mentioned but not cited (1)

  1. Structural advances toward understanding the catalytic activity and conformational dynamics of modular nonribosomal peptide synthetases. Patel KD, MacDonald MR, Ahmed SF, Singh J, Gulick AM. Nat Prod Rep 40 1550-1582 (2023)

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