PDBe 2fmm

X-ray diffraction
1.8Å resolution

Crystal Structure of EMSY-HP1 complex

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BRCA2-interacting transcriptional repressor EMSY Chain: E
Molecule details ›
Chain: E
Length: 133 amino acids
Theoretical weight: 14.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7Z589 (Residues: 9-139; Coverage: 10%)
Gene names: C11orf30, EMSY, GL002
Sequence domains: ENT domain
Structure domains: Methyltransferase, Methionine Synthase (B12-binding Domains); Chain A, domain 1
Chromobox protein homolog 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 74 amino acids
Theoretical weight: 8.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P83916 (Residues: 104-175; Coverage: 39%)
Gene names: CBX, CBX1
Sequence domains: Chromo shadow domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21212
Unit cell:
a: 143.4Å b: 63.87Å c: 66.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli