2flo

X-ray diffraction
2.2Å resolution

Crystal structure of exopolyphosphatase (PPX) from E. coli O157:H7

Released:

Function and Biology Details

Reaction catalysed:
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exopolyphosphatase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 524 amino acids
Theoretical weight: 60.21 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFL8 (Residues: 2-513; Coverage: 100%)
Gene names: ECs3364, Z3765, ppx
Sequence domains: Ppx/GppA phosphatase family
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C, NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 95.547Å b: 132.139Å c: 107.85Å
α: 90° β: 105.1° γ: 90°
R-values:
R R work R free
0.205 0.202 0.247
Expression system: Escherichia coli