2fln

X-ray diffraction
2.5Å resolution

binary complex of catalytic core of human DNA polymerase iota with DNA (template A)

Released:
Source organism: Homo sapiens
Entry authors: Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase iota Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 47.03 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9UNA4 (Residues: 26-445; Coverage: 57%)
Gene names: POLI, RAD30B
Sequence domains:
Structure domains:
DNA template strand Chain: T
Molecule details ›
Chain: T
Length: 11 nucleotides
Theoretical weight: 3.34 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA primer strand Chain: P
Molecule details ›
Chain: P
Length: 7 nucleotides
Theoretical weight: 2.09 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 98.114Å b: 98.114Å c: 202.823Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.226 0.279
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided