› 2fk2

X-ray diffraction
1.65Å resolution

Structure of the Alzheimer's Amyloid Precursor Protein (APP) Copper Binding Domain in 'small unit cell' form, Cu(I)-bound

Released:
DOI: 10.2210/pdb2fk2/pdb
PDB entry 2fk2 coloured by chain, front view.
PDB entry 2fk2 coloured by chain, side view.
PDB entry 2fk2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural studies of the Alzheimer's amyloid precursor protein copper-binding domain reveal how it binds copper ions.
Kong GK, Adams JJ, Harris HH, Boas JF, Curtain CC, Galatis D, Masters CL, Barnham KJ, McKinstry WJ, Cappai R, Parker MW
J Mol Biol 367 148-61 (2007)
PMID: 17239395

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138353 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-APP Chain: A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 6.85 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P05067 (Residues: 133-189; Coverage: 8%)
Gene names: A4, AD1, APP
Sequence domains: Copper-binding of amyloid precursor, CuBD
Structure domains: Amyloidogenic glycoprotein, copper-binding domain

Ligands and Environments

1 bound ligand:
Ligand CU1 1 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P212121
Unit cell:
a: 31.439Å b: 32.573Å c: 50.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.249
Expression system: Komagataella pastoris

Quick links

Citations

19 review citations

Metals in Alzheimer's and Parkinson's diseases.
Barnham et al. (2008)
18 more

2 mentions without citation

Copper binding to the Alzheimer's disease amyloid precursor protein.
Kong et al. (2008)
1 more