2fjg

X-ray diffraction
2.8Å resolution

Structure of the G6 Fab, a phage derived Fab fragment, in complex with VEGF

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero dodecamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Vascular endothelial growth factor A Chains: V, W
Molecule details ›
Chains: V, W
Length: 102 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15692 (Residues: 34-135; Coverage: 50%)
Gene names: VEGF, VEGFA
Sequence domains: PDGF/VEGF domain
Structure domains: Cystine-knot cytokines
Fab light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 227 amino acids
Theoretical weight: 24.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P3121
Unit cell:
a: 117.88Å b: 117.88Å c: 212.562Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.199 0.239
Expression system: Escherichia coli