› 2fj1

X-ray diffraction
2.2Å resolution

Crystal Structure Analysis of Tet Repressor (class D) in Complex with 7-Chlortetracycline-Nickel(II)

Released:
DOI: 10.2210/pdb2fj1/pdb
PDB entry 2fj1 coloured by chain, front view.
PDB entry 2fj1 coloured by chain, side view.
PDB entry 2fj1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Specific binding of divalent metal ions to tetracycline and to the Tet repressor/tetracycline complex.
Palm GJ, Lederer T, Orth P, Saenger W, Takahashi M, Hillen W, Hinrichs W
J Biol Inorg Chem 13 1097-110 (2008)
PMID: 18548290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACT4 (Residues: 2-208; Coverage: 95%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NI 1 x NI
Ligand CL 3 x CL
Ligand CTC 1 x CTC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: I4122
Unit cell:
a: 68.937Å b: 68.937Å c: 180.893Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.146 0.215
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
3 more

1 mention without citation

Tetracycline-tet repressor binding specificity: insights from experiments and simulations.
Aleksandrov et al. (2009)