› 2ffr

X-ray diffraction
2.03Å resolution

Crystallographic studies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine

Released:
DOI: 10.2210/pdb2ffr/pdb
PDB entry 2ffr coloured by chain, front view.
PDB entry 2ffr coloured by chain, side view.
PDB entry 2ffr coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Crystallographic studies on N-azidoacetyl-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine.
Petsalakis EI, Chrysina ED, Tiraidis C, Hadjiloi T, Leonidas DD, Oikonomakos NG, Aich U, Varghese B, Loganathan D
Bioorg Med Chem 14 5316-24 (2006)
PMID: 16616506

Function and Biology Details

Reaction catalysed: 
((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen phosphorylase, muscle form Chain: A
Molecule details ›
Chain: A
Length: 825 amino acids
Theoretical weight: 95.28 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P00489 (Residues: 13-837; Coverage: 98%)
Gene name: PYGM
Sequence domains: Carbohydrate phosphorylase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand DL6 1 x DL6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P43212
Unit cell:
a: 128.626Å b: 128.626Å c: 116.163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.199

Quick links

Citations

5 citation in other articles

Glucose-based spiro-heterocycles as potent inhibitors of glycogen phosphorylase.
Nagy et al. (2009)
4 more