Solution NMR

Solution structure of the complex between poxvirus-encoded CC chemokine inhibitor vCCI and human MIP-1beta, minimized average structure


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RPXV184 Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 26.16 KDa
Source organism: Rabbitpox virus
Expression system: Komagataella pastoris
  • Canonical: O10647 (Residues: 17-258; Coverage: 94%)
Gene names: RPXV001, RPXV184
Sequence domains: 35kD major secreted virus protein
Structure domains: Major secreted virus protein
C-C motif chemokine 4 Chain: B
Molecule details ›
Chain: B
Length: 69 amino acids
Theoretical weight: 7.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P13236 (Residues: 24-92; Coverage: 100%)
Gene names: CCL4, LAG1, MIP1B, SCYA4
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry simulated annealing
Expression systems:
  • Komagataella pastoris
  • Escherichia coli BL21(DE3)