X-ray diffraction
2.8Å resolution

Mutant R188M of The Cytidine Monophosphate Kinase from E. coli complexed with dCMP


Function and Biology Details

Reaction catalysed:
ATP + (d)CMP = ADP + (d)CDP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Cytidylate kinase Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 24.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P0A6I0 (Residues: 1-227; Coverage: 100%)
Gene names: JW0893, b0910, cmk, mssA, ycaF, ycaG
Sequence domains: Cytidylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P41212
Unit cell:
a: 72.949Å b: 72.949Å c: 76.96Å
α: 90° β: 90° γ: 90°
R R work R free
0.259 0.259 0.339
Expression system: Escherichia coli