2fed

X-ray diffraction
3.32Å resolution

Structure of the E203Q mutant of the Cl-/H+ exchanger CLC-ec1 from E.Coli

Released:
Source organisms:
Primary publication:
Separate ion pathways in a Cl-/H+ exchanger.
OpenAccess logo J Gen Physiol 126 563-70 (2005)
PMID: 16316975

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 465 amino acids
Theoretical weight: 49.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 1-465; Coverage: 98%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Fab fragment, heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab fragment, light chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 232.274Å b: 98.226Å c: 170.364Å
α: 90° β: 131.73° γ: 90°
R-values:
R R work R free
0.25 0.248 0.287
Expression systems:
  • Escherichia coli BL21(DE3)
  • Mus musculus