› 2f9j

X-ray diffraction
3Å resolution

3.0 angstrom resolution structure of a Y22M mutant of the spliceosomal protein p14 bound to a region of SF3b155

Released:
DOI: 10.2210/pdb2f9j/pdb
PDB entry 2f9j coloured by chain, front view.
PDB entry 2f9j coloured by chain, side view.
PDB entry 2f9j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a core spliceosomal protein interface.
Schellenberg MJ, Edwards RA, Ritchie DB, Kent OA, Golas MM, Stark H, Lührmann R, Glover JN, MacMillan AM
Proc Natl Acad Sci U S A 103 1266-71 (2006)
PMID: 16432215

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131049 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor 3B subunit 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 14.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Y3B4 (Residues: 1-125; Coverage: 100%)
Gene names: CGI-110, HSPC175, HT006, SAP14, SF3B14, SF3B14A, SF3B6
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Splicing factor 3B subunit 1 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 36 amino acids
Theoretical weight: 4.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75533 (Residues: 381-415; Coverage: 3%)
Gene names: SAP155, SF3B1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P41
Unit cell:
a: 71.271Å b: 71.271Å c: 102.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.266
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli

Quick links

Citations

13 review citations

The spliceosome: design principles of a dynamic RNP machine.
Wahl et al. (2009)
12 more

2 mentions without citation

Structure of the human activated spliceosome in three conformational states.
Zhang et al. (2018)
1 more