2f5q

X-ray diffraction
2.35Å resolution

Catalytically inactive (E3Q) MutM crosslinked to oxoG:C containing DNA CC2

Released:
Primary publication:
Structure of a DNA glycosylase searching for lesions.
Science 311 1153-7 (2006)
PMID: 16497933

Function and Biology Details

Reactions catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Formamidopyrimidine-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.71 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84131 (Residues: 1-274; Coverage: 100%)
Gene names: fpg, mutM
Structure domains:
5'-D(*AP*GP*G*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.95 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(*TP*GP*CP*G*TP*CP*CP*AP*(8OG)P*GP*TP*CP*TP*AP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P212121
Unit cell:
a: 44.413Å b: 92Å c: 103.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.249
Expression systems:
  • Escherichia coli
  • Not provided