› 2f4t

X-ray diffraction
3Å resolution

Asite RNA + designer antibiotic

Released:
DOI: 10.2210/pdb2f4t/pdb
PDB 2f4t coloured by chain and viewed from the front.
PDB 2f4t coloured by chain and viewed from the side.
PDB 2f4t coloured by chain and viewed from the top.
Source organism: Synthetic construct
Primary publication:
Interactions of designer antibiotics and the bacterial ribosomal aminoacyl-tRNA site.
Murray JB, Meroueh SO, Russell RJ, Lentzen G, Haddad J, Mobashery S
Chem Biol 13 129-38 (2006)
PMID: 16492561

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
5'-R(*GP*CP*GP*UP*CP*AP*CP*AP*CP*CP*GP*GP*UP*GP*AP*AP*GP*UP*CP*GP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.74 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(*UP*UP*GP*CP*GP*UP*CP*AP*CP*AP*CP*CP*GP*GP*UP*GP*AP*AP*GP*UP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.36 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand AB9 1 x AB9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 45.976Å b: 32.926Å c: 46.414Å
α: 90° β: 95.62° γ: 90°
R-values:
R R work R free
0.211 0.211 0.267
Expression system: Not provided

Quick links

Citations

5 review citations

Overcoming Aminoglycoside Enzymatic Resistance: Design of Novel Antibiotics and Inhibitors.
Zárate et al. (2018)
4 more

1 mention without citation

Novel insights of structure-based modeling for RNA-targeted drug discovery.
Chen et al. (2012)