› 2f2g

X-ray diffraction
2.1Å resolution

X-Ray Structure of Gene Product From Arabidopsis Thaliana AT3G16990

Released:
DOI: 10.2210/pdb2f2g/pdb
PDB entry 2f2g coloured by chain, front view.
PDB entry 2f2g coloured by chain, side view.
PDB entry 2f2g coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of gene locus At3g16990 from Arabidopsis thaliana.
Blommel PG, Smith DW, Bingman CA, Dyer DH, Rayment I, Holden HM, Fox BG, Phillips GN
Proteins 57 221-2 (2004)
PMID: 15326608

Function and Biology Details

Reaction catalysed: 
4-amino-5-aminomethyl-2-methylpyrimidine + H(2)O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional TENA-E protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.33 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ASY9 (Residues: 1-221; Coverage: 100%)
Gene names: At3g16990, K14A17.22, K14A17_11, TENA_E
Sequence domains: TENA/THI-4/PQQC family
Structure domains: Heme oxygenase-like

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand HMH 2 x HMH
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P41212
Unit cell:
a: 62.7Å b: 62.7Å c: 287.621Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.234
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cellular export of sugars and amino acids: role in feeding other cells and organisms.
Kim et al. (2021)
1 other article

1 mention without citation

Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine.
Zallot et al. (2014)