› 2ex4

X-ray diffraction
1.75Å resolution

Crystal Structure of Human methyltransferase AD-003 in complex with S-adenosyl-L-homocysteine

Released:
DOI: 10.2210/pdb2ex4/pdb
PDB 2ex4 coloured by chain and viewed from the front.
PDB 2ex4 coloured by chain and viewed from the side.
PDB 2ex4 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Entry authors: Min JR, Wu H, Zeng H, Loppnau P, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-[protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-terminal Xaa-Pro-Lys N-methyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 27.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BV86 (Residues: 2-223; Coverage: 100%)
Gene names: AD-003, C9orf32, METTL11A, NRMT, NRMT1, NTMT1
Sequence domains: AdoMet dependent proline di-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 43.922Å b: 47.367Å c: 64.734Å
α: 105.72° β: 88.18° γ: 115.97°
R-values:
R R work R free
0.197 0.194 0.258
Expression system: Escherichia coli

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