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X-ray diffraction
1Å resolution

The T24V mutant of tetraheme cytochrome c3 from Desulfovibrio Vulgaris Miyazaki F

Released:
DOI: 10.2210/pdb2ewk/pdb
PDB 2ewk coloured by chain and viewed from the front.
PDB 2ewk coloured by chain and viewed from the side.
PDB 2ewk coloured by chain and viewed from the top.
Source organism: Desulfovibrio vulgaris str. 'Miyazaki F'
Primary publication:
Strategic roles of axial histidines in structure formation and redox regulation of tetraheme cytochrome c3.
Takayama Y, Werbeck ND, Komori H, Morita K, Ozawa K, Higuchi Y, Akutsu H
Biochemistry 47 9405-15 (2008)
PMID: 18702516

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c3 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.56 KDa
Source organism: Desulfovibrio vulgaris str. 'Miyazaki F'
Expression system: Shewanella oneidensis
UniProt:
  • Canonical: P00132 (Residues: 24-130; Coverage: 100%)
Gene name: DvMF_2499
Sequence domains: Class III cytochrome C family
Structure domains: Cytochrome C3

Ligands and Environments

1 bound ligand:
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 52.425Å b: 67.537Å c: 34.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.112 0.11 0.14
Expression system: Shewanella oneidensis

Quick links

Citations

4 citation in other articles

Thermodynamic characterization of the redox centres in a representative domain of a novel c-type multihaem cytochrome.
Morgado et al. (2009)
3 more

1 mention without citation

The oxygen-oxygen distance of water in crystallographic data sets.
Palese LL. (2020)