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X-ray diffraction
2.68Å resolution

Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 5.3

Released:
DOI: 10.2210/pdb2ev3/pdb
PDB 2ev3 coloured by chain and viewed from the front.
PDB 2ev3 coloured by chain and viewed from the side.
PDB 2ev3 coloured by chain and viewed from the top.
Source organism: Mycobacterium tuberculosis
Primary publication:
The structure of the regulatory domain of the adenylyl cyclase Rv1264 from Mycobacterium tuberculosis with bound oleic acid.
Findeisen F, Linder JU, Schultz A, Schultz JE, Brügger B, Wieland F, Sinning I, Tews I
J. Mol. Biol. 369 1282-95 (2007)
PMID: 17482646

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
pH-sensitive adenylate cyclase Rv1264 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 24.17 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMU9 (Residues: 1-207; Coverage: 52%)
Gene names: MTCY50.18c, Rv1264
Sequence domains: Adenylate cyclase regulatory domain

Ligands and Environments

1 bound ligand:
Ligand OLA 2 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 120.403Å b: 52.976Å c: 89.399Å
α: 90° β: 133.73° γ: 90°
R-values:
R R work R free
0.231 0.229 0.265
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Acylation of Biomolecules in Prokaryotes: a Widespread Strategy for the Control of Biological Function and Metabolic Stress.
Hentchel et al. (2015)
4 more