› 2et6

X-ray diffraction
2.22Å resolution

(3R)-Hydroxyacyl-CoA Dehydrogenase Domain of Candida tropicalis Peroxisomal Multifunctional Enzyme Type 2

Released:
DOI: 10.2210/pdb2et6/pdb
PDB entry 2et6 coloured by chain, front view.
PDB entry 2et6 coloured by chain, side view.
PDB entry 2et6 coloured by chain, top view.
Source organism: Candida tropicalis
Primary publication:
Crystal structure of yeast peroxisomal multifunctional enzyme: structural basis for substrate specificity of (3R)-hydroxyacyl-CoA dehydrogenase units.
Ylianttila MS, Pursiainen NV, Haapalainen AM, Juffer AH, Poirier Y, Hiltunen JK, Glumoff T
J Mol Biol 358 1286-95 (2006)
PMID: 16574148

Function and Biology Details

Reactions catalysed: 
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisomal hydratase-dehydrogenase-epimerase Chain: A
Molecule details ›
Chain: A
Length: 604 amino acids
Theoretical weight: 65.56 KDa
Source organism: Candida tropicalis
Expression system: Escherichia coli
UniProt:
  • Canonical: P22414 (Residues: 1-604; Coverage: 67%)
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P212121
Unit cell:
a: 74.895Å b: 78.34Å c: 95.445Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.252
Expression system: Escherichia coli

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Citations

1 review citation

The structures of type I polyketide synthases.
Keatinge-Clay AT. (2012)
10 other articles

2 mentions without citation

Sea Anemones Responding to Sex Hormones, Oxybenzone, and Benzyl Butyl Phthalate: Transcriptional Profiling and in Silico Modelling Provide Clues to Decipher Endocrine Disruption in Cnidarians.
Morgan et al. (2021)
1 more