PDBe 2eq7

X-ray diffraction
1.8Å resolution

Crystal structure of lipoamide dehydrogenase from thermus thermophilus HB8 with psbdo

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Nakai T, Kamiya N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dihydrolipoyl dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 49.13 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLK6 (Residues: 1-455; Coverage: 100%)
Gene name: TTHA0287
Sequence domains:
Structure domains:
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex Chain: C
Molecule details ›
Chain: C
Length: 40 amino acids
Theoretical weight: 4.35 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided
UniProt:
  • Canonical: Q5SLK5 (Residues: 101-140; Coverage: 10%)
Gene name: TTHA0288

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand NAD 2 x NAD
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 85.028Å b: 107.073Å c: 131.706Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.238
Expression systems:
  • Escherichia coli
  • Not provided