› 2ei1

X-ray diffraction
1.52Å resolution

Anaerobic Crystal Structure Analysis of the 1,2-dihydroxynaphthalene dioxygeanse of Pseudomonas sp. strain C18 complexes to 1,2-dihydroxynaphthalene

Released:
DOI: 10.2210/pdb2ei1/pdb
PDB entry 2ei1 coloured by chain, front view.
PDB entry 2ei1 coloured by chain, side view.
PDB entry 2ei1 coloured by chain, top view.
Source organism: Pseudomonas sp. C18
Entry authors: Neau DB, Kelker MS, Colbert CL, Bolin JT

Function and Biology Details

Reaction catalysed: 
Naphthalene-1,2-diol + O(2) = 2-hydroxy-2H-chromene-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,2-dihydroxynaphthalene dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 33.99 KDa
Source organism: Pseudomonas sp. C18
Expression system: Pseudomonas putida
UniProt:
  • Canonical: P0A108 (Residues: 1-302; Coverage: 100%)
Gene name: doxG
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

4 bound ligands:
Ligand FE2 1 x FE2
Ligand MG 1 x MG
Ligand D1N 1 x D1N
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I422
Unit cell:
a: 117.848Å b: 117.848Å c: 120.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.18
Expression system: Pseudomonas putida

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