› 2efw

X-ray diffraction
2.5Å resolution

Crystal structure of the RTP:nRB complex from Bacillus subtilis

Released:
DOI: 10.2210/pdb2efw/pdb
PDB entry 2efw coloured by chain, front view.
PDB entry 2efw coloured by chain, side view.
PDB entry 2efw coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
An asymmetric structure of the Bacillus subtilis replication terminator protein in complex with DNA.
Vivian JP, Porter CJ, Wilce JA, Wilce MC
J Mol Biol 370 481-91 (2007)
PMID: 17521668

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115206 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Replication termination protein Chains: A, B, F, G
Molecule details ›
Chains: A, B, F, G
Length: 122 amino acids
Theoretical weight: 14.53 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0CI76 (Residues: 1-122; Coverage: 100%)
Gene names: BSU18490, rtp
Sequence domains: Replication terminator protein
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*DCP*DT*DAP*DTP*DGP*DTP*DAP*DCP*DCP*DAP*DAP*DAP*DTP*DGP*DTP*DTP*DCP*DAP*DGP*DTP*DC)-3') Chains: D, I
Molecule details ›
Chains: D, I
Length: 21 nucleotides
Theoretical weight: 6.4 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
DNA (5'-D(*DGP*DAP*DCP*DTP*DGP*DAP*DAP*DCP*DAP*DTP*DTP*DTP*DGP*DGP*DTP*DAP*DCP*DAP*DTP*DAP*DG)-3') Chains: E, J
Molecule details ›
Chains: E, J
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 124.012Å b: 129.626Å c: 125.221Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.293 0.291 0.332
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

Mechanisms of polar arrest of a replication fork.
Kaplan et al. (2009)
1 more

3 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
2 more