› 2ecs

X-ray diffraction
1.4Å resolution

Lambda Cro mutant Q27P/A29S/K32Q at 1.4 A in space group C2

Released:
DOI: 10.2210/pdb2ecs/pdb
PDB entry 2ecs coloured by chain, front view.
PDB entry 2ecs coloured by chain, side view.
PDB entry 2ecs coloured by chain, top view.
Source organism: Escherichia virus Lambda
Primary publication:
Two structures of a lambda Cro variant highlight dimer flexibility but disfavor major dimer distortions upon specific binding of cognate DNA.
Hall BM, Roberts SA, Heroux A, Cordes MH
J Mol Biol 375 802-11 (2008)
PMID: 18054042

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-136477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein cro Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.36 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03040 (Residues: 1-66; Coverage: 100%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor

Ligands and Environments

4 bound ligands:
Ligand SO4 4 x SO4
Ligand ACT 4 x ACT
Ligand CL 1 x CL
Ligand LI 2 x LI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 88.689Å b: 26.999Å c: 55.523Å
α: 90° β: 103.69° γ: 90°
R-values:
R R work R free
0.141 0.139 0.175
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Current View on EpCAM Structural Biology.
Gaber et al. (2020)
8 other articles

2 mentions without citation

The protein interaction map of bacteriophage lambda.
Rajagopala et al. (2011)
1 more