› 2e91

X-ray diffraction
2.14Å resolution

S. cerevisiae geranylgeranyl pyrophosphate synthase in complex with magnesium and BPH-91

Released:
DOI: 10.2210/pdb2e91/pdb
PDB entry 2e91 coloured by chain, front view.
PDB entry 2e91 coloured by chain, side view.
PDB entry 2e91 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases.
Guo RT, Cao R, Liang PH, Ko TP, Chang TH, Hudock MP, Jeng WY, Chen CK, Zhang Y, Song Y, Kuo CJ, Yin F, Oldfield E, Wang AH
Proc Natl Acad Sci U S A 104 10022-7 (2007)
PMID: 17535895

Function and Biology Details

Reactions catalysed: 
Prenyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranylgeranyl pyrophosphate synthase BTS1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 39.3 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12051 (Residues: 1-335; Coverage: 100%)
Gene names: BTS1, YPL069C
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ZOL 2 x ZOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P212121
Unit cell:
a: 46.657Å b: 116.293Å c: 126.946Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Terpenoid synthase structures: a so far incomplete view of complex catalysis.
Gao et al. (2012)
26 more

3 mentions without citation

Structural Insight into Geranylgeranyl Diphosphate Synthase (GGDPS) for Cancer Therapy.
Pham et al. (2024)
2 more