2dwn

X-ray diffraction
3.35Å resolution

Crystal structure of the PriA protein complexed with oligonucleotides

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Primosomal protein N' Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 11.78 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17888 (Residues: 1-105; Coverage: 14%)
Gene names: JW3906, b3935, priA
Sequence domains: 3'DNA-binding domain (3'BD)
Structure domains: PriA, 3(prime) DNA-binding domain
DNA (5'-D(*A*G)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 2 nucleotides
Theoretical weight: 597 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: R32
Unit cell:
a: 112.25Å b: 112.25Å c: 260.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.281 0.281 0.333
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided