X-ray diffraction
3.2Å resolution

Crystal structure of the PriA protein complexed with oligonucleotides


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Primosomal protein N' Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 11.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P17888 (Residues: 1-105; Coverage: 14%)
Gene names: JW3906, b3935, priA
Sequence domains: 3'DNA-binding domain (3'BD)
Structure domains: PriA, 3(prime) DNA-binding domain
5'-D(*AP*(DC))-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 2 nucleotides
Theoretical weight: 557 Da
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: R32
Unit cell:
a: 111.51Å b: 111.51Å c: 260.46Å
α: 90° β: 90° γ: 120°
R R work R free
0.247 0.247 0.302
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided