› 2due

X-ray diffraction
1.24Å resolution

crystal structure of a green fluorescent protein variant S65T/H148D at pH 10

Released:
DOI: 10.2210/pdb2due/pdb
PDB 2due coloured by chain and viewed from the front.
PDB 2due coloured by chain and viewed from the side.
PDB 2due coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Entry authors: Shu X, Remington SJ

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.91 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand C12 1 x C12

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 50.887Å b: 62.59Å c: 69.911Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.148 0.204
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Low barrier hydrogen bonds in protein structure and function.
Kemp et al. (2021)
9 more

1 mention without citation

Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 1. Mutagenesis and structural studies.
Shu et al. (2007)