› 2drp

X-ray diffraction
2.8Å resolution

THE CRYSTAL STRUCTURE OF A TWO ZINC-FINGER PEPTIDE REVEALS AN EXTENSION TO THE RULES FOR ZINC-FINGER/DNA RECOGNITION

Released:
DOI: 10.2210/pdb2drp/pdb
PDB entry 2drp coloured by chain, front view.
PDB entry 2drp coloured by chain, side view.
PDB entry 2drp coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The crystal structure of a two zinc-finger peptide reveals an extension to the rules for zinc-finger/DNA recognition.
Fairall L, Schwabe JW, Chapman L, Finch JT, Rhodes D
Nature 366 483-7 (1993)
PMID: 8247159

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115186 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein tramtrack, beta isoform Chains: A, D
Molecule details ›
Chains: A, D
Length: 66 amino acids
Theoretical weight: 8.04 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P17789 (Residues: 501-563; Coverage: 10%)
Gene names: CG1856, FTZ-F2, ttk
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(*CP*TP*AP*AP*TP*AP*AP*GP*GP*AP*TP*AP*AP*CP*GP*TP*C P*CP*G)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*TP*CP*GP*GP*AP*CP*GP*TP*TP*AP*TP*CP*CP*TP*TP*AP*T P*TP*A)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 19 nucleotides
Theoretical weight: 5.79 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 60.7Å b: 64.6Å c: 117.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

39 review citations

Genome editing with engineered zinc finger nucleases.
Urnov et al. (2010)
38 more

22 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
21 more