2dpm

X-ray diffraction
1.8Å resolution

DPNM DNA ADENINE METHYLTRANSFERASE FROM STREPTOCCOCUS PNEUMONIAE COMPLEXED WITH S-ADENOSYLMETHIONINE

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Modification methylase DpnIIA Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 32.95 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P04043 (Residues: 1-284; Coverage: 100%)
Gene name: dpnM
Sequence domains: D12 class N6 adenine-specific DNA methyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 56.6Å b: 68.1Å c: 85.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.284
Expression system: Escherichia coli