2d9q

X-ray diffraction
2.8Å resolution

Crystal Structure of the Human GCSF-Receptor Signaling Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Granulocyte colony-stimulating factor Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 18.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09919 (Residues: 31-207; Coverage: 98%)
  • Best match: P09919-2 (Residues: 31-204)
Gene names: C17orf33, CSF3, GCSF
Sequence domains: Granulocyte colony-stimulating factor
Structure domains: Growth Hormone; Chain: A;
Granulocyte colony-stimulating factor receptor Chain: B
Molecule details ›
Chain: B
Length: 313 amino acids
Theoretical weight: 34.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q99062 (Residues: 25-332; Coverage: 38%)
Gene names: CSF3R, GCSFR
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P3221
Unit cell:
a: 134.777Å b: 134.777Å c: 105.468Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.251 0.284
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda