PDBe 2d5r

X-ray diffraction
2.5Å resolution

Crystal Structure of a Tob-hCaf1 Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CCR4-NOT transcription complex subunit 7 Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 29.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UIV1 (Residues: 11-262; Coverage: 88%)
Gene names: CAF1, CNOT7
Sequence domains: CAF1 family ribonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H
Protein Tob1 Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 13.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50616 (Residues: 1-115; Coverage: 33%)
Gene names: TOB, TOB1, TROB1
Sequence domains: BTG family
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I422
Unit cell:
a: 151.505Å b: 151.505Å c: 114.181Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.246
Expression system: Escherichia coli