X-ray diffraction
2.3Å resolution

Crystal structure of the thermostable Japanese Firefly Luciferase complexed with MgATP


Function and Biology Details

Reaction catalysed:
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Luciferin 4-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 548 amino acids
Theoretical weight: 60.13 KDa
Source organism: Luciola cruciata
Expression system: Escherichia coli
  • Canonical: P13129 (Residues: 1-548; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 57.78Å b: 181.975Å c: 53.566Å
α: 90° β: 90° γ: 90°
R R work R free
0.182 0.179 0.228
Expression system: Escherichia coli