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› 2cv2

X-ray diffraction
2.69Å resolution

Glutamyl-tRNA synthetase from Thermus thermophilus in complex with tRNA(Glu) and an enzyme inhibitor, Glu-AMS

Released:
DOI: 10.2210/pdb2cv2/pdb
PDB entry 2cv2 coloured by chain, front view.
PDB entry 2cv2 coloured by chain, side view.
PDB entry 2cv2 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Structural bases of transfer RNA-dependent amino acid recognition and activation by glutamyl-tRNA synthetase.
Sekine S, Shichiri M, Bernier S, Chênevert R, Lapointe J, Yokoyama S
Structure 14 1791-9 (2006)
PMID: 17161369

Function and Biology Details

Reaction catalysed: 
[tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate., tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate.]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-302746 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Glutamate--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 53.99 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27000 (Residues: 1-468; Coverage: 100%)
Gene names: TTHA0438, gltX
Sequence domains:
Structure domains:
tRNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 nucleotides
Theoretical weight: 24.11 KDa
Source organism: Thermus thermophilus
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand CL 2 x CL
Ligand GSU 2 x GSU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2221
Unit cell:
a: 110.317Å b: 219.822Å c: 135.831Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Experimental and computational determination of tRNA dynamics.
Alexander et al. (2010)
1 more

7 mentions without citation

Personalized Medicine in Mitochondrial Health and Disease: Molecular Basis of Therapeutic Approaches Based on Nutritional Supplements and Their Analogs.
Tragni et al. (2022)
6 more