2cu0

X-ray diffraction
2.1Å resolution

Crystal structure of inosine-5'-monophosphate dehydrogenase from Pyrococcus horikoshii OT3

Released:
Source organism: Pyrococcus horikoshii
Entry authors: Asada Y, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed:
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 486 amino acids
Theoretical weight: 53 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O58045 (Residues: 1-486; Coverage: 100%)
Gene names: PH0307, guaB
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I4
Unit cell:
a: 123.666Å b: 123.666Å c: 130.053Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.228
Expression system: Escherichia coli