› 2co7

X-ray diffraction
1.8Å resolution

Salmonella enterica SafA pilin in complex with the SafB chaperone (Type II)

Released:
DOI: 10.2210/pdb2co7/pdb
PDB entry 2co7 coloured by chain, front view.
PDB entry 2co7 coloured by chain, side view.
PDB entry 2co7 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism.
Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G
Mol Cell 22 831-842 (2006)
PMID: 16793551

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187380 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Saf-pilin pilus formation protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.36 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZRK4 (Residues: 44-170; Coverage: 88%)
Gene names: STM0299, safA
Sequence domains: Saf-pilin pilus formation protein
Structure domains: Dr adhesin
Fimbriae assembly chaparone Chain: B
Molecule details ›
Chain: B
Length: 221 amino acids
Theoretical weight: 24.02 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZRK3 (Residues: 17-237; Coverage: 93%)
Gene names: STM0300, safB
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 114.388Å b: 40.595Å c: 72.039Å
α: 90° β: 95.82° γ: 90°
R-values:
R R work R free
0.192 0.19 0.228
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
29 more

4 mentions without citation

Natural Products Attenuating Biosynthesis, Processing, and Activity of Ras Oncoproteins: State of the Art and Future Perspectives.
Tisi et al. (2020)
3 more