› 2chi

X-ray diffraction
1.6Å resolution

Recombinant human H ferritin, K86Q and E27D mutant

Released:
DOI: 10.2210/pdb2chi/pdb
PDB entry 2chi coloured by chain, front view.
PDB entry 2chi coloured by chain, side view.
PDB entry 2chi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution X-ray structures of human apoferritin H-chain mutants correlated with their activity and metal-binding sites.
Toussaint L, Bertrand L, Hue L, Crichton RR, Declercq JP
J Mol Biol 365 440-52 (2007)
PMID: 17070541

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 21.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02794 (Residues: 1-183; Coverage: 100%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand ZN 2 x ZN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: F432
Unit cell:
a: 180.528Å b: 180.528Å c: 180.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.193
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Intracellular iron transport and storage: from molecular mechanisms to health implications.
MacKenzie et al. (2008)
9 more

3 mentions without citation

Cryo-EM single-particle structure refinement and map calculation using Servalcat.
Yamashita et al. (2021)
2 more