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Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family.

Thaw P, Sedelnikova SE, Muranova T, Wiese S, Ayora S, Alonso JC, Brinkman AB, Akerboom J, van der Oost J, Rafferty JB
Nucleic Acids Res 34 1439-49 (2006)
Cited: 64 times
EuropePMC logo PMID: 16528101

Abstract

The Lrp/AsnC family of transcriptional regulatory proteins is found in both archaea and bacteria. Members of the family influence cellular metabolism in both a global (Lrp) and specific (AsnC) manner, often in response to exogenous amino acid effectors. In the present study we have determined both the first bacterial and the highest resolution structures for members of the family. Escherichia coli AsnC is a specific gene regulator whose activity is triggered by asparagine binding. Bacillus subtilis LrpC is a global regulator involved in chromosome condensation. Our AsnC-asparagine structure is the first for a regulator-effector complex and is revealed as an octameric disc. Key ligand recognition residues are identified together with a route for ligand access. The LrpC structure reveals a stable octamer supportive of a topological role in dynamic DNA packaging. The structures yield significant clues to the functionality of Lrp/AsnC-type regulators with respect to ligand binding and oligomerization states as well as to their role in specific and global DNA regulation.

Articles - 2cg4 mentioned but not cited (4)

  1. Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family. Thaw P, Sedelnikova SE, Muranova T, Wiese S, Ayora S, Alonso JC, Brinkman AB, Akerboom J, van der Oost J, Rafferty JB. Nucleic Acids Res 34 1439-1449 (2006)
  2. Crystal structure of glutamine receptor protein from Sulfolobus tokodaii strain 7 in complex with its effector L-glutamine: implications of effector binding in molecular association and DNA binding. Kumarevel T, Nakano N, Ponnuraj K, Gopinath SC, Sakamoto K, Shinkai A, Kumar PK, Yokoyama S. Nucleic Acids Res 36 4808-4820 (2008)
  3. Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit. Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W. Protein Sci 16 1360-1367 (2007)
  4. The structure, function and properties of sirohaem decarboxylase--an enzyme with structural homology to a transcription factor family that is part of the alternative haem biosynthesis pathway. Palmer DJ, Schroeder S, Lawrence AD, Deery E, Lobo SA, Saraiva LM, McLean KJ, Munro AW, Ferguson SJ, Pickersgill RW, Brown DG, Warren MJ. Mol Microbiol 93 247-261 (2014)


Reviews citing this publication (6)

  1. Bacterial nucleoid-associated proteins, nucleoid structure and gene expression. Dillon SC, Dorman CJ. Nat Rev Microbiol 8 185-195 (2010)
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  6. Alanine dehydrogenases in mycobacteria. Jeong JA, Oh JI. J Microbiol 57 81-92 (2019)

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  18. Crystal structure of the archaeal heat shock regulator from Pyrococcus furiosus: a molecular chimera representing eukaryal and bacterial features. Liu W, Vierke G, Wenke AK, Thomm M, Ladenstein R. J Mol Biol 369 474-488 (2007)
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  38. HU of Streptococcus pneumoniae Is Essential for the Preservation of DNA Supercoiling. Ferrándiz MJ, Carreño D, Ayora S, de la Campa AG. Front Microbiol 9 493 (2018)
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