› 2cb4

X-ray diffraction
2.5Å resolution

Crystal structure of the catalytic domain of the mosquitocidal toxin from Bacillus sphaericus, mutant E197Q

Released:
DOI: 10.2210/pdb2cb4/pdb
PDB entry 2cb4 coloured by chain, front view.
PDB entry 2cb4 coloured by chain, side view.
PDB entry 2cb4 coloured by chain, top view.
Source organism: Lysinibacillus sphaericus
Primary publication:
Structure of the mosquitocidal toxin from Bacillus sphaericus.
Reinert DJ, Carpusca I, Aktories K, Schulz GE
J Mol Biol 357 1226-36 (2006)
PMID: 16483607

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin B lectin domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 291 amino acids
Theoretical weight: 33.15 KDa
Source organism: Lysinibacillus sphaericus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q03988 (Residues: 30-308; Coverage: 32%)
Gene name: mtx1
Structure domains: Heat-Labile Enterotoxin, subunit A

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 70 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 123.71Å b: 143.267Å c: 135.814Å
α: 90° β: 100.58° γ: 90°
R-values:
R R work R free
0.175 0.174 0.194
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Bacillus sphaericus mosquitocidal toxin (MTX) and pierisin: the enigmatic offspring from the family of ADP-ribosyltransferases.
Carpusca et al. (2006)
14 other articles

5 mentions without citation

ADP-ribosylation systems in bacteria and viruses.
Mikolčević et al. (2021)
4 more