› 2ca6

X-ray diffraction
2.2Å resolution

MIRAS structure determination from hemihedrally twinned crystals

Released:
DOI: 10.2210/pdb2ca6/pdb
PDB entry 2ca6 coloured by chain, front view.
PDB entry 2ca6 coloured by chain, side view.
PDB entry 2ca6 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Detecting and overcoming hemihedral twinning during the MIR structure determination of Rna1p.
Hillig RC, Renault L
Acta Crystallogr D Biol Crystallogr 62 750-65 (2006)
PMID: 16790931

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ran GTPase-activating protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 43.27 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P41391 (Residues: 1-386; Coverage: 100%)
Gene names: SPAC22E12.07, rna1
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: I41
Unit cell:
a: 175.21Å b: 175.21Å c: 55.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.218
Expression system: Escherichia coli BL21

Quick links

Citations

2 citation in other articles

Crystal structure of the leucine-rich repeat domain of the NOD-like receptor NLRP1: implications for binding of muramyl dipeptide.
Reubold et al. (2014)
1 more

1 mention without citation

Characterization of the genome, proteome, and structure of yersiniophage ϕR1-37.
Skurnik et al. (2012)