X-ray diffraction
2.1Å resolution

Structure and activity of a flavonoid 3-0 glucosyltransferase reveals the basis for plant natural product modification


Function and Biology Details

Reaction catalysed:
UDP-D-glucose + an anthocyanidin = UDP + an anthocyanidin-3-O-beta-D-glucoside
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Anthocyanidin 3-O-glucosyltransferase UFGT Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 50.19 KDa
Source organism: Vitis vinifera
Expression system: Escherichia coli
  • Canonical: P51094 (Residues: 1-456; Coverage: 100%)
Gene names: AlUFGT1, AlUFGT2, FlUFGT1, FlUFGT2, GSVIVT00014047001, ITUFGT1, ITUFGT2, LOC100233099, RUUFGT1, RUUFGT2, UFGT, VITISV_008354, VVGT1
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 49.189Å b: 93.817Å c: 106.092Å
α: 90° β: 90° γ: 90°
R R work R free
0.2 0.198 0.235
Expression system: Escherichia coli