› 2c62

X-ray diffraction
1.74Å resolution

Crystal Structure of the Human Transcription Cofactor PC4 in Complex with Single-Stranded DNA

Released:
DOI: 10.2210/pdb2c62/pdb
PDB entry 2c62 coloured by chain, front view.
PDB entry 2c62 coloured by chain, side view.
PDB entry 2c62 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A global transcription cofactor bound to juxtaposed strands of unwound DNA.
Werten S, Moras D
Nat Struct Mol Biol 13 181-2 (2006)
PMID: 16415882

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115136 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Activated RNA polymerase II transcriptional coactivator p15 Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53999 (Residues: 63-127; Coverage: 51%)
Gene names: PC4, RPO2TC1, SUB1
Sequence domains: Transcriptional Coactivator p15 (PC4)
Structure domains: Transcriptional Coactivator Pc4; Chain A
5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP *TP*TP*TP*TP*TP*TP*TP*TP*TP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.06 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 67.242Å b: 67.242Å c: 131.899Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Antisense technology: A review.
Crooke et al. (2021)
3 more

11 mentions without citation

Visualizing single-stranded nucleic acids in solution.
Plumridge et al. (2017)
10 more