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Structural analysis of the interaction between the SNARE Tlg1 and Vps51.

Fridmann-Sirkis Y, Kent HM, Lewis MJ, Evans PR, Pelham HR
Traffic 7 182-90 (2006)
Related entries: 2c5i, 2c5k

Cited: 39 times
EuropePMC logo PMID: 16420526

Abstract

Membrane fusion in cells involves the interaction of SNARE proteins on apposing membranes. Formation of SNARE complexes is preceded by tethering events, and a number of protein complexes that are thought to mediate this have been identified. The VFT or GARP complex is required for endosome-Golgi traffic in yeast. It consists of four subunits, one of which, Vps51, has been shown to bind specifically to the SNARE Tlg1, which participates in the same fusion event. We have determined the structure of the N-terminal domain of Tlg1 bound to a peptide from the N terminus of Vps51. Binding depends mainly on residues 18-30 of Vps51. These form a short helix which lies in a conserved groove in the three-helix bundle formed by Tlg1. Surprisingly, although both Vps51 and Tlg1 are required for transport to the late Golgi from endosomes, removal of the Tlg1-binding sequences from Vps51 does not block such traffic in vivo. Thus, this particular interaction cannot be crucial to the process of vesicle docking or fusion.

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  5. Organization of SNAREs within the Golgi stack. Malsam J, Söllner TH. Cold Spring Harb Perspect Biol 3 a005249 (2011)
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  8. Traffic from the endosome towards trans-Golgi network. Saimani U, Kim K. Eur J Cell Biol 96 198-205 (2017)
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  18. Structural basis for the binding of SNAREs to the multisubunit tethering complex Dsl1. Travis SM, DAmico K, Yu IM, McMahon C, Hamid S, Ramirez-Arellano G, Jeffrey PD, Hughson FM. J Biol Chem 295 10125-10135 (2020)
  19. Dissecting Ent3p: the ENTH domain binds different SNAREs via distinct amino acid residues while the C-terminus is sufficient for retrograde transport from endosomes. Zimmermann J, Chidambaram S, Fischer von Mollard G. Biochem J 431 123-134 (2010)
  20. Crystal structure of Sec10, a subunit of the exocyst complex. Chen J, Yamagata A, Kubota K, Sato Y, Goto-Ito S, Fukai S. Sci Rep 7 40909 (2017)
  21. Importance of the N-terminal domain of the Qb-SNARE Vti1p for different membrane transport steps in the yeast endosomal system. Gossing M, Chidambaram S, Fischer von Mollard G. PLoS One 8 e66304 (2013)
  22. The inner workings of intracellular heterotypic and homotypic membrane fusion mechanisms. Cruz MD, Kim K. J Biosci 44 91 (2019)
  23. A computational prediction of structure and function of novel homologue of Arabidopsis thaliana Vps51/Vps67 subunit in Corchorus olitorius. Zaman A, Fancy NN. Interdiscip Sci 4 256-267 (2012)
  24. Structure of a membrane tethering complex incorporating multiple SNAREs. DAmico KA, Stanton AE, Shirkey JD, Travis SM, Jeffrey PD, Hughson FM. Nat Struct Mol Biol (2024)


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